P 373 adenoviral mediated β-galactosidase (lacZ) reporter gene expression in the mouse retina
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منابع مشابه
P 373 adenoviral mediated β-galactosidase (lacZ) reporter gene expression in the mouse retina
Pupose:In order to develop a treatment for recessive retinitis pigmentosa the ultimate objective is to introduce a normal copy of the abnormal gene into photoreceptors. The post-mitotic status of photoreceptor cells make them unsuitable targets for existing retroviral vectors. One of the strategies that we have been exploiting is adenoviral (AV) mediated gene transfer. Here we report successful...
متن کاملIn vivo transfer of a reporter gene to the retina mediated by an adenoviral vector.
PURPOSE The ability of replication-deficient adenovirus to mediate gene transfer to retinal cells was evaluated. METHODS A replication-deficient adenoviral vector, AdCMV beta A.ntlacZ, which contains the bacterial beta-galactosidase (lacZ) reporter gene, was injected into the subretinal space of normal, rd, and rds strains of mice at various ages. The efficiency and duration of transgene expr...
متن کاملlacZ transgenic rats tolerant for beta-galactosidase: recipients for gene transfer studies using lacZ as a reporter gene.
Gene transfer of reporter genes may trigger immune responses against the heterologous protein resulting in shortening of gene expression and inflammation. We generated transgenic rats expressing the lacZ gene under the control of the human immunodeficiency virus type 1 (HIV-1) long-terminal repeat (LTR) (HIV-lacZ) to obtain rats with undetectable transgene expression using histologic methods, t...
متن کاملExpression Cloning of Recombinant Escherichia coli lacZ Genes Encoding Cytoplasmic and Nuclear P-galactosidase Variants
Objective(s) Nonviral vector can be an attractive alternative to gene delivery in experimental study. In spite of some advantages in comparison with the viral vectors, there are still some limitations for efficiency of gene delivery in nonviral vectors. To determine the effective expression, the recombinant Escherichia coli lacZ genes were cloned into the different variants of pcDNA3.1 and the...
متن کاملAn artifact in studies of gene regulation using β-galactosidase reporter gene assays.
Reporter gene assays are important tools for evaluating gene expression. A frequently used assay measures the activity of β-galactosidase (β-gal) expressed from lacZ in plasmid or genomic constructions. Such constructions are often used to interrogate the ability of DNA (query DNA), potentially encoding a transcription factor, to regulate in trans the expression of a promoter fused to the repor...
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ژورنال
عنوان ژورنال: Vision Research
سال: 1995
ISSN: 0042-6989
DOI: 10.1016/0042-6989(95)90633-9